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Colorimetric detection of GSH based on the pFeSAN In the standard procedure, the assays were performed in 1 mL HAc-NaAc buffer (0.1 M, pH 4.0) with different concentrations of GSH (0.05, 0.5, 2.5, 5, 12.5, 25, 50, 100, 150, 200, 250, 500, or 1000 μM), where the TMB and pFeSAN concentrations were 1 mM and 20 μg/mL, respectively
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Moreover, genes that showed reduced chromatin association were deregulated in PHF3 ΔSPOC according to RNA-seq (Supplementary Fig