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Nonetheless, and regardless of the partitioning mechanism, the introduction of cholesterol in phospholipid membranes containing saturated and unsaturated lipids typically results in lipid phase separation into domains with different packing states, with tighter lipid packing in the L O domains and looser packing in the L D regions
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116,117 It is a homo-dimeric transmembrane protein whose extracellular domain can connect with the other same molecules of neighboring endothelial cells in the paracellular cleft, and the cytoplasmic domain can interact with the actin filaments via scaffolding proteins