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All environmental models, both biological (e.g., viral hypothesis) and biochemical (e.g., toxic hypothesis), focus on oxidative stress and consequently neuroinflammation as a common part of these concepts [129,140,162]

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After 3 washing intervals 100 µL enzyme conjugate (peroxidase labeled anti-CRP) was added on each micro well for additional 15 minutes incubation in room temperature in dark