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163 Additionally, VEGF-A stimulates its own negative regulator, sVEGFR1, through the VEGFR2-PKC-MEK pathway, establishing a feedback loop that limits VEGF-A activity and angiogenesis

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To measure the activity of glutathione peroxidase, 0.1 mL of 0.1 mL of brain homogenate and 20 mm tert-butyl hydroperoxide, incubated for 10 minutes at a temperature of 37 °C, the reaction is stopped with 0.02 mL of a solution of 25% trichloroacetic acid